Mass Spectrometry

The Department possesses a VG Autospec high resolution mass spectrometer. The instrument has an EBE geometry and can be operated at 8 KV up to 4500 Daltons. It has a programmable solid probe and flowing FAB (fast atom bombardment) and GC (gas chromatographic) inlets. A variety of ionization methods are available. FAB (fast atom bombardment) can be carried out with either a xenon or cesium gun. Positive and negative ion modes are accessible. The system is controlled by a DEC Alpha computer.

The Department also has a new Bruker Reflex III time-of-flight (TOF) mass spectrometer. MALDI (matrix-assisted laser desorption ionization) is used to vaporize and ionize samples, which are generally low-volatility compounds such as biomolecules, polymers, and inorganic materials. The mass spectrometer has PSD (post-source decay) capabilities that can be used to sequence biomolecules. The MALDI/TOF has a 364-well sample target and is controlled by a PC.The mass spectrometry facility is maintained by a full-time Ph.D. spectroscopist, who is available for consultation and assistance.

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