University of Alabama

Registration for Biohazardous Agents - Class 2

Investigator  ________________________________________________________________

Building(s)  ________________________________         Room(s)  ____________________ 

Dept  ____________________________________           Date  _______________________

Biosafety Level 2 (BSL-2) is similar to BSL-1 and is suitable for work involving agents of moderate potential hazard to personnel and the environment.  It differs from BSL-1 in that (1) laboratory personnel have had specific training in handling pathogenic agents and are directed by competent scientists; (2) access to the laboratory is limited when work is being conducted; (3) extreme precautions are taken with contaminated sharps items; and (4) certain procedures in which infectious aerosols or splashes may be created are conducted in biological safety cabinets or other physical containment equipment.  Standard microbiological practices and Universal Precautions apply to all work done with BSL-2 agents.  The lab has some special designs and features.

A biosafety manual must be prepared and be made readily available to all lab workers.  This biosafety plan must summarize the hazards of the work and describe the safety precautions and emergency procedures to be followed in the event of a spill or release.  The plan should be included in the initial training for new lab workers and should be reviewed with all workers annually. 

WRITTEN SAFETY PLAN

Agent Information:  Please give the following information on the agent/agents in the lab area. 

                Name:

                Epidemiology of diseases caused by or associated with the agent:

                Disease symptoms:

                Hazard Assessment:

                Check all the apply:    Human Hazard _____    Animal Hazard _____    Plant Hazard _____

Usage Information:  Briefly summarize how Class 2 agent(s) are to be used or investigated.  If available, you can attach copies of grants, publications, protocols, etc., that describe intended usage in detail.

Safety Precautions:  List all the safety practices and handling procedures which all laboratory personnel are required to follow.

                General Safety Practices (other than standard practices and universal precautions):

                Required Personal Protective Equipment/Clothing:

                Procedures requiring a Biological Safety Cabinet:

                Decontamination of work surface:

                Decontamination of equipment:

                Disposal of waste:

**Some BSL-2 pathogens can require BSL-3 containment when large quantities are used or when there is a high potential for aerosol production.  Please check if you will be using BSL-3 containment in your work.               ____________

Human Components:  If human blood components, body fluids or tissues are used, additional concerns may arise.  Please list below the specific substances to be used, the source of substances, and how they will be used.

                Have all new personnel had Bloodborne Pathogen Training?  Yes _____    No _____    NA ____  If not, contact EHS at 348-5905 to schedule training.

                Have all existing personnel had annual retraining on Bloodborne Pathogens?  Yes _____   No_____  If not, contact EHS at 348-5905 to schedule retraining.

Disposal Methods:  Please describe the disposal methods to be used for any waste containing infectious agents, biological toxins, or human blood components, fluids or tissues. 

Decontamination Practices:  Please describe the decontamination methods used for work surfaces and equipment.  If using a chemical disinfectant, please state type and concentration of product.

Emergency Procedures:  Describe the procedures to be followed in the event of personnel exposure or spills.

As LBSO, I certify that the above information is correct to the best of my knowledge and that I will follow the procedures outlined in the UA Biological Safety Manual as well as prudent microbiological safety and research practices.  I further certify that I will train all students and lab workers on this plan as well as on the practices and procedures required for their work.

Name of LBSO (printed):

Date:

Signature: