Spring 2005
Harriett E. Smith-Somerville, Professor

Office: 417 Biology Building
Lab: 416 Biology Building
Telephone: 348-1830

e-mail: hsmithso@ua.edu

Office hours: Tuesday, 3:30-4:30 PM

Dr. Kim Lackey, Laboratory Coordinator

Optical Analysis Facility: 101 Biology Building
Telephone: 348-1789

e-mail: lacke003@ua.edu

Prerequisites: Graduate standing
Course Goals

The goals of the course are

  • To introduce students to microscopical methods and applications in biological research, including techniques for preparation of biological specimens, operation of light microscopes, transmission and scanning electron microscopes and the confocal laser scanning microscope, and production and presentation of micrographs.
  • To provide students with sufficient background and training so that they may incorporate microscopical techniques into their individual research plans.
Student Learning Outcomes

Upon completion of this course, students will be

  1. Familiar with the principles of microscope design and operation
  2. Able to prepare specimens for different applications and microscopes
  3. Able to operate the microscopes and ancillary equipment
  4. Able to critically discriminate between reliable and less reliable information based on microscopic observations
Textbooks
  • Electron Microscopy, Principles and Techniques for Biologists, 2nd Edition, 1999, John J. Bozzola and Lonnie D. Russell, Jones & Bartlett Publishers
  • Fundamentals of Light Microscopy and Electronic Imaging, 2001, Douglas B. Murphy, Wiley-Liss
  • Supplemental material assigned in class
Course Information and Communications

Course information, schedule, instructions, and notices will be posted in eLearning

Attendance Policy

Attendance of class discussions and laboratories is required.  Students are responsible for scheduling appointments for training on equipment.  A missed scheduled appointment without prior notification or a very good excuse (very sick with doctor's excuse, death in the family) will result in dismissal from the course.  Poor planning of experiments does not count as an excuse.

Grading
Discussion: 25% The discussion grade will be determined by participation in class discussions and written answers to questions. Questions and due dates are listed under "Questions" in WebCT. The grade will be lowered by one letter grade for each day work is submitted past the date due.
Laboratory: 75% The laboratory grade will be based on a notebook that you will hand in on the day of the scheduled final exam and on participation and performance in the laboratory. The grade will be lowered by one letter grade for each day work is submitted past the date due.
Notebook

The notebook will include:

  • Details of sample preparation for each sample submitted
  • Three or more negatives and corresponding prints from designated specimens with magnification of each negative and each print. Requirement may be modified for particular specimens
  • A discussion of problems encountered in sample preparation and micrographs
  • A discussion of how these problems might be corrected
Disability

If you are registered with the Office of Disability Services, please make an appointment with me as soon as possible to discuss any course accommodations that may be necessary.  If you have a disability but have not contacted the Office of Disability Services, please call 348-4285 or visit 133B Martha Parham East to register for services.

Academic Misconduct

All acts of dishonesty in any work constitute academic misconduct.  This includes, but is not limited to, cheating, plagiarism, fabrication of information, misrepresentations, and abetting of any of the above.  The Academic Misconduct Disciplinary Policy will be followed in the event that academic misconduct occurs.  Students should refer to the Student Affairs Handbook, which can be obtained in The Office of Student Life and Services in the Ferguson Center.

Updated December 17, 2007