Date

Topic

Assignment for Discussion

January

9

Introduction

*Electron Microscopy 1
**Light Microscopy 2
Light and Color

 

14

Light & Resolution

Electron Microscopy 6: 149-156
Light Microscopy 5, 6: 85-90
Light Microscopy
Microscopy Primer
Basic Concepts in Optical Microscopy
Basic Concepts and Formulas in Microscopy
The Nature of Electromagnetic Radiation

Numerical Aperture and Resolution
Refraction of Light
Resolution
Interference
Harmonics and Patterns

 

16

Lens Design, Aberrations, Depth of Field & Focus, Magnification

Electron Microscopy 6: 156-162
Light Microscopy 4: 43-52, 6: 90-91
Optical Aberrations
Microscopy Primer
Microscope Objectives

Magnification

 

21

Martin Luther King, Jr. Day

 

23

Light Microscope, Specimen Preparation

 Light Microscopy 1, 3, 4: 53-60, 6: 91-95
Anatomy of a Microscope
Microscopy Primer

 

28

Enhancing Visibility

Light Microscopy 7-10
Contrast Enhancing Techniques

Polarized Light Microscopy

 

30

TEM Design

Electron Microscopy 6: 163-179

February

4

TEM Design

Electron Microscopy 6: 179-188

 

6

TEM Operation

Electron Microscopy 6: 188-201, 16

 

11

SEM Design

Electron Microscopy 7: 203-219

 

13

SEM 3-D, Modes of Operation

Electron Microscopy 7: 219-239

 

18

TEM Specimen Preparation
Question 1 Due

Electron Microscopy 2: 17-34

 

20

TEM Specimen Preparation

Electron Microscopy 2: 34-47

 

25

Ultramicrotomy

Electron Microscopy 4: 73-97

 

27

Ultramicrotomy

Electron Microscopy 4: 97-118

March

3

Contrast Methods for TEM

Electron Microscopy 5

 

5

Freeze Fracture Replication

Electron Microscopy 14

 

10

SEM Specimen Preparation

Electron Microscopy 3: 49-58

 

12

SEM Specimen Preparation

Electron Microscopy 3: 58-71

 

17

Spring Break

 

19

Spring Break

 

24

Photography Using Film

Electron Microscopy 8: 242-256
Introduction to Photomicrography

 

26

Printing

Electron Microscopy 8: 250-256

31

Digital Photography

Electron Microscopy 18: 415-437
Light Microscopy
: 14, 15
Digital Imaging

  April

2

Illustrations for Publication And Presentation

Electron Microscopy  8: 256-261
Light Microscopy
: 16

 

7

Specimen Preparation Using Antibodies
Question 2 Due

 Electron Microscopy  9: 263-280

 

9

Fluorescence Microscopy

Light Microscopy 11
Fluorescence Microscopy
Fluorescence Tutorials

 

14

Confocal & Deconvolution Microscopes

Light Microscopy 12
Theory of Confocal Microscopy
Confocal Imaging
Digital Confocal Microscopy
Optical Sectioning

Spinning Disc Confocal Microscope
Deconvolution in Optical Microscopy

 

16

Other Localization Techniques, Tracers

Electron Microscopy 10, 12, 17

 

21

Autoradiography

Electron Microscopy 11

 

23

Quantitative Electron Microscopy
Question 3 Due

Electron Microscopy 13, 18: 437-441

 

28

Analytical Electron Microscopy

Electron Microscopy 15

 

30

Problems & Interpretation

Electron Microscopy 19

May

7

Notebook due by 5 PM

 

*Electron Microscopy, Principles and Techniques for Biologists, 2nd Edition, 1999, John J. Bozzola and Lonnie D. Russell, Jones and Bartlett Publishers
**
    Fundamentals of Light Microscopy and Electronic Imaging, 2001, Douglas B. Murphy, Wiley-Liss
Updated January 16, 2008