Baits

Click on this link to see enlarged images of the baits

The kinds of baits used for isolating chytrids from gross culture include pine pollen, insect exuviae (shed exoskeleton), processed shrimp chitin, white onion skin, hemp rope, hemp seeds, snake skin, boiled grass leaves, boiled algae, cellophane (usually in the form of dialysis tubing), corn husks, also known as corn straw, defatted blond baby hair, rosaceous fruits like rose hips, lens paper, and birch twigs. This is by no means an exhaustive listing of possible baits. Reading Sparrow's Aquatic Phycomycetes is a good way to find out what kind of bait to use as he gives a nice overview of collection and isolation techniques in the introduction and lists the host/substrate from which a particular chytrid was isolated with each species description. It also helps to read the scientific literature to see what kinds of baits researchers are using in their own work.

How to prepare certain baits for isolation:

Here is a picture of two bait bags made from mosquito netting and sewn together with regular sewing thread using a zigzag stitch. The bags are closed with a drawstring to keep the baits and marbles from floating out.