9th INTERNATIONAL CONFERENCE ON PARTITIONING IN AQUEOUS
TWO-PHASE SYSTEMS
Advances in the Uses of Polymers in Cell Biology, Biotechnology and
Environmental Sciences
Scientific Program
Sunday, 4th June
16,30 - 18,30 Registration
18,30 - 19,00 Opening Remarks
Monday, 5th June
(09,00 - 11,00) Lectures
APPLICATIONS OF PARTITIONING/POLYMERS IN BIOTECHNOLOGY
Chairpersons F. Tjerneld and T. Franco
09,05 - APPLICATION OF POLYOXYETHYLENE DETERGENT-BASED AQUEOUS
TWO-PHASE SYSTEMS FOR DOWNSTREAM PROCESSING OF AMPHIPHILE PROTEINS.
T. Minuth, J. Thmmes and M.-R. Kula.
09,25 - USE OF CHAOTROPIC AQUEOUS TWO-PHASE SYSTEMS FOR THE
RECOVERY OF RECOMBINANT PROTEINS. T. Spears and D. Forciniti.
09,45 - AQUEOUS TWO-PHASE SYSTEMS AS A SAMPLE PREPARATION METHOD
PRIOR POLYMERASE CHAIN REACTION. P.G. Lantz, Matsson, F. Tjerneld, E. Borch, T.
Wadstrm and P Radstrm.
10,05 - CHITINASE FERMENTATION WITH SERRATIA MARCENSENS IN AN
AQUEOUS TWO-PHASE SYSTEM. Jyh-Ping Chen and Maw-Shyan Lee.
10,25 - EFFECT OF DIFFERENT AQUEOUS TWO-PHASE SYSTEMS ON
LACTOCOCCUS AND LACTOBACILLUS FOR LACTIC ACID PRODUCTION. J. Planas, P.
Radstrm, F. Tjerneld and B. Hahn-Hgerdal.
(11,30 - 11,50) Poster Presentation
APPLICATIONS OF PARTITIONING/POLYMERS IN BIOTECHNOLOGY
Chairman F. Tjerneld
P#1 - PRACTICAL IMPLEMENTATION OF AQUEOUS TWO-PHASE PARTITION
PROCESSES. M. Rito-Palomares, Jon G. Huddleston and Andrew Lydiatt.
P#2 - AQUEOUS TWO-PHASE PARTITION OF COMPLEX PROTEIN FEEDSTOCKS
DERIVED FROM TISSUE HOMOGENATES. Simon G. Walker, Christopher J. Dale and Andrew
Lydiatt.
P#3 - SYNTHETIC AND NATURAL MACROMOLECULES USING IN
BIOTECHNOLOGY AS SUPPORTS FOR IMMOBILIZATION INDUSTRIAL ENZYMES.
Luminita Tcacenco, Steliana Maxim, Irina Lupescu.
P#4 - FTIR AND ACTIVITY STUDIES ON THE EFFECTS OF POLAR ORGANIC
SOLVENTS OF PROTEINS. Sorana Fraier, Vlad Brumfeld, Smadar Cohen.
(11,55 - 12,15) Poster Presentation
PARTITIONING OF MACROMOLECULES: BASIC PRINCIPLES
Chairman G. Johansson
P#5 - EFFECTS OF SALTS AND SURFACE HYDROPHOBICITY OF PROTEINS ON
PARTITIONING IN AQUEOUS TWO-PHASE SYSTEMS CONTAINING
THERMOSEPARATING ETHYLENE OXIDE COPOLYMERS. Kristina Axelsson, Hans-Olof
Johansson and Folke Tjerneld.
P#6 - PARTITIONING OF AMINO ACIDS, PEPTIDES AND PROTEINS IN UCON-
WATER AQUEOUS TWO-PHASE SYSTEMS H-O Johansson, G. Karlstrm, F. Tjerneld.
P#7 - CRYOPARTITIONING OF ENZYMES PRESENT IN YEAST EXTRACT. Bndicte
Grimonprez and Gte Johansson.
(12,15 - 13,00) Poster Viewing and Discussion
(15,00 - 16,30) Lectures
PARTITIONING OF MACROMOLECULES: BASIC PRINCIPLES
Chairpersons G. Johansson and J.A. Asenjo
15,00 - PARTITIONING OF ENZYMES IN TWO-PHASE SYSTEMS WITH ONE
PROTEIN-RICH PHASE. Gte Johansson.
15,30 - MODEL FOR PREDICTING THE PARTITION BEHAVIOUR OF PROTEINS IN
AQUEOUS TWO-PHASE SYSTEMS. Andrews, B.A., Schmidt, A.S. and Asenjo, J.A.
15,50 - PHASE BEHAVIOUR AND PROTEIN PARTITIONING IN WEAKLY
CHARGED POLYMER-SURFACTANT-WATER MIXTURES. Karin Bergfeldt, Lennart Piculell,
Ulf Sivars and Folke Tjerneld.
16,10 - PROTEIN PARTITION BETWEEN THE DIFFERENT COMPRISING PEG-SALT
AQUEOUS TWO-PHASE SYSTEMS, HYDROPHOBIC INTERACTION OF
CHROMATOGRAPHY AND PRECIPITATION: A GENERIC DESCRIPTION IN TERMS OF
SALTING EFFECTS. Jonathan Huddleston, Rayduen Wang, Loan Carles Abelaira and Andrew
Lyddiatt.
(17,00 - 18,30) Lectures
PREDICTION OF PARTITIONING
Chairman H. Cabezas
17,00 - FUNDAMENTAL STUDIES OF PARTITIONING OF RECOMBINANT
PROTEINS IN PEG-DEXTRAN AQUEOUS TWO-PHASE SYSTEMS A. Veide, K. Axelsson, M.
Carlsson, P. Linse and F. Tjerneld.
17,30 - A MATHEMATICAL MODEL FOR THE PARTITIONING OF PEPTIDES AND
PEPTIDE-MODIFIED PROTEINS IN AQUEOUS TWO-PHASE SYSTEMS. Mark A. Eiteman,
Cynthia Hassinen, Andrs Veide.
17,50 - MATHEMATICAL MODELLING AND COMPUTER SIMULATION OF
CONTINOUS AQUEOUS TWO-PHASE PROTEIN EXTRACTION. S.L. Mistry, F. Addo-Yobo,
J.A. Asenjo and J.C. Merchuck.
Tuesday, 6th June
(09,00 - 11,00) Lectures
METAL EXTRACTION BY PARTITIONING: APPLICATIONS IN
ENVIRONMENTAL SCIENCES
Chairpmen Robin D. Rogers and Roberto Guzman
09,00 - MODELING ION PARTITIONING IN AQUEOUS TWO-PHASE SYSTEMS.
Heriberto Cabezas.
09,50 - SYNTHESIS AND CHARACTERIZATION OF SOLUBLE CHELATING-
POLYMERS AND THEIR APPLICATION IN AQUEOUS TWO-PHASE SYSTEMS. R. Guzm n,
Porath, P. Agui¤aga and J. Garc”a.
10,10 - METAL ION SEPARATION IN POLYETHYLENE GLYCOL-BASED AQUEOUS
BIPHASIC SYSTEMS: THE OUTLOOK FOR COMMERCIAL APPLICATIONS. Robin D.
Rogers, Andrew H. Bond, Cary B. Bauer, Jianhua Zhang and Scott T. Griffin.
- METAL ION SEPARATIONS IN POLYETHYLENE GLYCOL-BASED AQUEOUS
BIPHASIC SYSTEMS: CORRELATION OF PARTITIONING BEHAVIOUR WITH
AVAILABLE THERMODYNAMIC HYDRATION DATA. Robin D. Rogers, Andre H. Bond, Cary
B. Bauer, Jianhua Zhang.
(11,30 - 12,15) Poster Presentation
METAL EXTRACTION BY PARTITIONING: APPLICATIONS IN
ENVIRONMENTAL SCIENCES
Chairman Robin D. Rogers
P#8 - PARTITIONING BEHAVIOUR OF GROUP 1 AND 2 CATIONS IN AQUEOUS
BIPHASIC SYSTEMS: THE DEVELOPMENT OF PREDICTIVE MODELS BASED ON
THERMODYNAMIC HYDRATION DATA. Cary B. Bauer and Robin D. Rogers.
P#9 - EFFECTS OF INCREASING POLYMER HYDROPHOBICITY ON DISTRIBUTION
RATIOS OF TcO4- IN POLYETHYLENE/POLYPROPYLENE GLYCOL-BASED AQUEOUS
BIPHASIC SYSTEMS. Jianhua Zhang and Robin D. Rogers.
P#10 - THE INFLUENCE OF THE pH DIFFERENCE O N PARTITIONING
PEG/CITRATE AQUEOUS TWO-PHASE SYSTEMS. Mark A. Eiteman.
P#11 - RECOVERY OF METAL IONS FROM SOLUTIONS USING WATER SOLUBLE
CHELATING-POLYMERS AND AQUEOUS TWO-PHASE SYSTEMS. Pablo Aguinaga, Carlos
Tellez, Francisco Ruiz, Javier Garcia and Roberto Guzman.
(12,00 - 12,25) Poster Presentation
BIOMEDICAL APPLICATION OF POLYMERS
Chairman L.W. Seymour
P#13 - PRODUCTION OF INTERLEUKIN-1 BY MAMMALIAN CELLS CULTURED
WITHIN ALGINATE HYDROGELS. Dganit Bar, Nilli Grossman, Ron N. Apte and Smadar Cohen.
P#14 - ARA-C-TRAPPING INTO PHEMA HYDROGELS: IN VITRO AND IN VIVO
DRUG DELIVERY STUDIES. J.M. Teij¢n, R.M. Trigo, J. Socorro, P. Huerta and M.D. Blanco.
P#15 - 5-FLUOROACIL RELEASE FROM POLY(ACRYLAMIDE-co-MONOPROPYL
ITACONATE) HYDROGELS. M.D. Blanco, O. Garc”a, R. Olmo and I. Katime.
P#16 - MONOCLONAL ANTIBODY PRODUCTION FROM HYDROGEL-
ENTRAPPED HYBRIDOMA CELLS CULTIVATED IN AIR-LIFT REACTOR. Amira Trevgoda-
Weisman, Jose C. Merchuk and Smadar Cohen.
(12,25 - 13,00) Poster Viewing and Discussion
(15,00 - 16,30) Lectures
BIOMEDICAL APPLICATION OF POLYMERS
Chairpersons L.W. Seymour and D. Fisher
15,00 - SOLUBLE POLYMERS FOR IMPROVED DELIVERY OF DRUGS. L.W. Seymour.
15,35 - PEGYLATION OR TRANSFERRIN FOR CLINICAL USE. Angel Herr ez, Farooq
Malik, Dolores Briones, Derek Fisher, Gillian E. Francis and Cristina Delgado.
16,00 - PREPARATION OF PEG-GM-CSF CONJUGATES WITH PEGs OF
INCREASING LENGTH: CHARACTERIZATION BY PHASE PARTITIONING AND GEL
PERMEATION CHROMATOGRAPHY. Farooq Malik, Derek Fisher, Gillian E. Francis and
Cristina Delgado.
(17,00 - 18,30) Lectures
STRUCTURAL AND INTERACTION STUDIES
Chairpersons G. Birkenmeier and D. Forciniti
17,00 - EFFECTS OF DIFFERENT PEPTIDE INSERTIONS ON PROTEIN ADSORPTION
PROPERTIES. Andres Veide and Martin Malmsten.
17,20 - EFFICIENCY OF SEPARATION OF METAL IONS AND PROTEINS IN
AQUEOUS TWO-PHASE SYSTEMS. USING SOLUBLE CHELATING POLYMERS. R.
Guzm n, J. Porath, P. Agui¤aga and J. Garc”a.
17,40 - LIQUID-LIQUID PARTITION CHROMATOGRAPHY AS A METHOD TO
EXAMINE CONFORMATIONAL CHANGES OCCURRING UPON BINDING OF LIGAND
BY BIOMOLECULES. C. Wingren, U-B. Hansson and K. Andersson.
18,00 - USE OF SMALL-ANGLE NEUTRON SCATTERING FOR THE STUDY OF
PROTEIN-PROTEIN INTERACTIONS. P. Petit and D. Forciniti.
Wednesday, 7th June
(09,00 - 09,30) Poster Presentation
STRUCTURAL AND INTERACTION STUDIES
Chairman G. Birkenmeier
P#17 - ELECTROKINETIC ANALYSIS OF POLYMER COATED SURFACES. J.M. Van
Alstine, J.M. Harris, N.L. Burns, K. Emoto and K. Holmberg.
P#18 - SURFACE BEHAVIOUR OF POLY(ETHYLENE GLYCOL) AMPHIPHILES AND
THE USE OF PEG-LIGANDS IN PHASE PARTITION. J.M. Van Alstine and Martin Malmsten.
P#19 - PARTITION COEFFICIENT AND POLYMER MODIFICATION OF PROTEINS.
C. Delgado and James M. Van Alstine.
P#20 - LIGAND INTERACTION OF HUMAN ALPHA 2-MACROGLOBULIN/ALPHA
2-MACROGLOBULIN RECEPTOR STUDIES BY PARTITIONING IN AQUEOUS TWO-
PHASE SYSTEMS. M. Kunath, G. Birkenmeier and G. Kopperschlger.
P#21 - EVALUATION OF VISCOSITIES OF POLYMER/WATER SOLUTIONS USED
IN ATPS. Ufuk Gnduz.
P#22 - LIQUID-LIQUID PARTITION CHROMATOGRAPHY IN AQUEOUS TWO-
PHASE SYSTEMS A UNIQUE TOOL FOR PROTEIN ANALYSES. U.-B. Hansson and C.
Wingren.
(09,40 - 10,10) Poster Viewing and Discussion
(10,30 - 12,00) PLENARY LECTURE IN HONOUR OF PROF. P.. ALBERTSSON AND
PROF. H. WALTER
Chairman G. Johansson
Speakers F. Tjerneld and D. Fisher
Thursday, 8th June
(09,00 - 11,00) Lectures
PARTITIONING OF CELLS, ORGANELLES AND PLASMA MEMBRANES
Chairpersons C. Larsson and H. Walter
09,00 - FORMATION OF SEALED INSIDE-OUT PLASMA MEMBRANE VESICLES
FROM RIGHT-SIDE-OUT VESICLES USING POLYOXYTEHYLENE ETHERS AND ESTERS.
C. Larsson, F. Johansson, M. Olbe, G. Johansson and M. Sommarin.
09,40 - PURIFICATION AND FUNCTIONAL PROPERTIES OF INSIDE-OUT PLASMA
MEMBRANE VESICLES FROM SACCHAROMYCES CEREVISIAE Amaia Menndez, Christer
Larsson and Unai Ugalde.
10,00 - AQUEOUS TWO-PHASE PARTITIONING AND PREPARATIVE FREE-FLOW
ELECTROPHORESIS IN TANDEM FOR RAT MUSCLE FRACTIONATION S. Moya-
Camarena, J. Lawrence, W.T. Garvey, L. Mainau, D.M. Morr and D.J. Morr.
10,20 - AQUEOUS TWO-PHASE PARTITIONING: IMAP AN ALTERNATIVE
METHOD OF CELL STUDY AND PURIFICATION. E. Laboreau, K. Chadha and M.A.
Vijayalakshmi.
10,40 - REVERSION OF THERMIC-SHOCK EFFECT ON RAM SPERMATOZOA BY
ADSORPTION OF SEMINAL PLASMA PROTEINS REVEALED BY PARTITION IN
AQUEOUS TWO-PHASE SYSTEMS. Garc”a-L¢pez, N., Ollero, M., Cebri n-Prez, J.A. and Mui¤o-
Blanco, T.
(11,30 - 12,10) Poster Presentation
PARTITIONING OF CELLS, ORGANELLES AND PLASMA MEMBRANES
Chairman C. Larsson
P#23 - TRANSMEMBRANE DISTRIBUTION OF PHOSPHOLIPIDS IN SPINACH
THYLAKOID INSIDE-OUT VESICLES AND INVOLVEMENT OF OUTER AND INNER
MONOLAYER PHOSPHOLIPIDS IN THE PHOTOSYNTHETIC ELECTRON FLOW
ACTIVITY. S. Dchene, J. Smutny and P.-A. Siegenthaler.
P#24 - PURIFICATION OF PEA NODULE SYMBIOSOMES USING AN AQUEOUS
POLYMER TWO-PHASE SYSTEM. L.E. Hern ndez, P. Rojas, D.T. Cooke and R. Carpena-Ruiz.
P#25 - PROGRESS TOWARD GOLGI APPARATUS ISOLATION FROM CULTURED
MAMMALIAN CELLS AIDED BY PARTITIONING IN AQUEOUS TWO-PHASE. S. Moya-
Camarena, D.J. Morr and D.M. Morr.
P#26 - HETEROGENEITY OF HYPOTONICALLY LOADED RAT ERYTHROCYTE
POPULATIONS AS DETECTED BY COUNTER-CURRENT DISTRIBUTION IN AQUEOUS
POLYMER TWO-PHASE SYSTEMS. M. Teresa Prez, Ana-Isabel Grac”a-Prez, F. Javier Alvarez,
Luisa Lucas, M. Cristina Tejedor and Pilar Sancho.
P#27 - AMPHIPATIC SURFACE PROPERTIES OF HUMAN PLATELET DISCOCYTES
AND ECHINOCYTES. James M. Van Alstine, Basil Chiu, Patricia A. Alred and Donald E. Brooks.
P#28 - STUDY OF HUMAN CORD BLOOD LYMPHOCYTES BY IMAP
(IMMOBILIZED METAL ION AFFINITY PARTITIONING). E. Laboreau, J.C. Capiod, C.
Dessaint, L. Print and M.A. Vijayalakshmi.
P#29 - SURFACE CHANGES ASSOCIATED TO SPERM CRYOPRESERVATION.
EFFECT OF DIFFERENT CRYOPROTECTANTS. Ollero, M., Mui¤o-Blanco, T., L¢pez-Prez, M.J.
and Cebri n-Prez, J.A.
(12,15 - 12,35) Poster Presentation
PURIFICATION OF MACROMOLECULES
Chairwoman N. Moreau
P#30 - SURFACTANT BASED EXTRACTION OF DETERGENT ENZYMES. Edit L.
Braunstein, Nathaniel T. Becker and Grant Ganshaw.
P#31 - ENZYME PURIFICATION WITH AQUEOUS TWO-PHASE SYSTEMS:
COMPARISON BETWEEN SYSTEMS COMPOSED BY PURE POLYMERS AND SYSTEMS
COMPOSED BY CRUDE POLYMERS. A. Venncio, C. Almeida and J.A. Teixeira.
P#32 - FRACTIONATION OF WHEAT PROTEINS USING COUNTER-CURRENT
DISTRIBUTION WITH A PROPIONIC ACID CONTAINING AQUEOUS TWO-PHASE
SYSTEM. Helle Truust and Gte Johansson.
P#33 - RNA PARTITIONING IN SALT/PEG TWO-PHASE SYSTEM. K. Kimura and H.
Kobayashi.
(12,35 - 13,00) Poster Viewing and Discussion
(15,00 - 16,30) Lectures
PURIFICATION OF MACROMOLECULES
Chairpersons N. Moreau and A. S nchez-Ferrer
15,05 - PURIFICATION OF OXIDASES IN PLANT EXTRACTS CONTAINING A HIGH
LEVEL OF PHENOLS BY TRITON X-114. E. Nu¤ez-Delicado, R. Bru, A. S nchez-Ferrer and F.
Garc”a-Carmona.
15,25 - ENHANCING PROTEIN PARTITIONING TO THE PEG PHASE USING BIO-
SPECIFIC LIPOSOMES IN AQUEOUS TWO-PHASE SYSTEM. Naomi Moldavski and Smadar
Cohen.
15,45 - PURIFICATION AND CHARACTERIZATION OF GAMMA-CRYSTALLINS
USING PEG/DX SYSTEMS. D. Forciniti.
16,05 - PROTEIN PURIFICATION USING AQUEOUS TWO-PHASE SYSTEMS AND
RECOVERY OF PHASE FORMING POLYMERS. Min Lu, Per-ke Albertsson, Gte Johansson
and Folke Tjerneld.
Friday, 9th June
(09,00 - 11,00) Lectures
PHASE SEPARATION AND NEW POLYMERS
Chairman P.. Albertsson
09,00 - KINETICS OF PHASE SEPARATION OF PEG-PHOSPHATE TWO-PHASE
SYSTEMS: EXPERIMENTAL STUDIES AND MODELLING. Asenjo, J.A., Kaul, A., Mistry, S.L.
and Merchuk, J.C.
09,40 - PARTITIONING AND PHASE BEHAVIOUR IN AQUEOUS TWO-PHASE
SYSTEMS CONTAINING MICELLE-FORMING BLOCK COPOLYMERS. Mrten Svensson,
Per Linse and Folke Tjerneld.
10,00 - THE BEHAVIOUR OF POLYSACCHARIDES OF BRAZILIAN LEGUMINOUS
SPECIES ON THE FORMATION OF AQUEOUS TWO-PHASE SYSTEMS. Telma T. Franco, M.
Estela Silva, Wladimir M. Gordo, Valris R. Panegassi, Gil E. Serra and Marcos Buckeridge.
10,20 - COUNTERCURRENT DISTRIBUTION ON THE SPACEHAB 1 SHUTTLE
MISSION. James M. Van Alstine.
(11,30 - 13,00) ROUND TABLE ON POTENTIAL APPLICATIONS OF PHASE
SYSTEMS
Chairman J.M. Van Alstine
(13,00) CLOSING REMARKS